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However, integrative analysis using multiple datasets are few. ![]() A recent study also investigated therapeutic targets in BPH using single-cell RNA-seq (scRNA-seq) data. Functional activity and related pathways of biomarkers including BMP5, NELL2, IGF1, CXCL13, MXRA5 and GPX3 were identified from many analyses using gene microarrays and RNA-seq datasets. With the development of bioinformatics analysis in recent years, many studies have given new insight into pathophysiology mechanism of BPH. Therefore, fibrosis and EMT might be a promising therapeutic target for BPH/LUTS. EMT is characterized as increasing mesenchymal-like cells derived from the prostatic epithelium and TGF-β is thought to play a key role in EMT. Meanwhile, epithelial-mesenchymal transition (EMT) also contributes to prostatic fibrosis and hyperplasia, which leads to BPH/LUTS progress. Fibrosis was proposed to be a new pathological mechanism of BPH and involved in the progress of LUTS, however, the current first-line oral medications (α1-blockers and 5-ARIs) have shown no effect on reverse or alleviation of fibrosis. Stroma myofibroblasts play pivotal role in the process of fibrosis that accumulation of which results in remodeling of the extracellular matrix (ECM) and tissue hardening by secreting large amounts of collagen proteins deposited. However, from the pathological aspect, besides the continuous nonmalignant proliferation of prostate cells, BPH tissues also exhibit stromal fibrosis. With regard to the pathogenesis of BPH/LUTS, some hypothesis have been proposed including growth factor, stromal-epithelial interaction, ratio between estrogen and androgen, chronic inflammation, genetic and familial factors, metabolic syndrome, autoimmunity and embryonic reawakening theory. To date, aging and testosterone are two defined independently risk factor of BPH/LUTS. Further and in-depth of investigations for therapeutic targets are of great significances. The dilemma in medical therapy of BPH/LUTS was probably due to its not-fully revealed etiology and pathogenesis. ![]() Despite current medical therapy such as α1-adrenoceptor antagonists (α1-blockers) and 5α-reductase inhibitors (5-ARIs) serve satisfactory, a portion of patients might experience complications or require further surgical interventions. NRK may play important roles in the development of BPH and may be a promising therapeutic target for BPH/LUTS.īenign prostatic hyperplasia (BPH) and its related lower urinary tract symptoms (LUTS) are very common in elder men, which affecting their quality of life. Our novel data identified NRK was upregulated in hyperplastic prostate and associated with prostatic stromal cell proliferation, apoptosis, cell cycle, migration, fibrosis and EMT process. Furthermore, expression level of NRK was positively correlated with that of α-SMA, collagen-I and N-cadherin, negatively correlated with that of E-cadherin. Meanwhile, induced fibrosis and EMT process were rescued by knockdown of NRK. Silencing of NRK inhibited stromal cell proliferation, migration, fibrosis and EMT process, promoted apoptosis and induced cell cycle arrest, while overexpression of NRK in prostate epithelial cells showed opposite results. The expression level of NRK was significantly correlated with IPSS and Q max of BPH patients. Meanwhile, NRK was upregulated in BPH samples and localized almost in stroma. The findings of integrated single-cell analysis showed that NRK remarkably upregulated in fibroblasts and SM cells of hyperplasia prostate. ResultsĪ total of 17 DEGs were identified by merging three bulk RNA-seq datasets. The localization, expression and functional activity of NRK was investigated via RT-PCR, western-blot, immunohistochemical staining, flow cytometry, wound healing assay, transwell assay and CCK-8 assay. Cell clusters and specific metabolism pathways were analyzed. ![]() Methodsįour datasets including three bulk and one single cell RNA-seq (scRNA-seq) were obtained to perform integrated bioinformatics. The current study aims to identify differentially expressed genes (DEGs) in hyperplastic prostate and to explore the role of Nik related kinase (NRK) in BPH. Benign prostatic hyperplasia (BPH) is common in elder men. ![]() ![]() ![]() 10: Rebuild the file headerĪlthough every Word document is different, Word documents that are created by a common version of Word have a common file header. For more on the shadow copy feature, see How do I… Configure and use shadow copy in Microsoft Windows Vista?. (This tab exists only for files stored on NTFS volumes.) The Previous Versions tab will show you any previous versions of the file that are available. When the document’s properties sheet appears, select the Previous Versions tab. To do so, right-click on the document and select the Properties command from the shortcut menu. If a shadow copy of your document exists, it may be possible to recover a recent version of the document. Windows Vista automatically saves shadow copies of some files to the hard drive. 9: Use a Vista shadow copy of the document This isn’t necessarily foolproof, but it does work on a lot of problematic documents. Just select the file in the Open dialog box and choose Open And Repair from the Open drop-down list in the bottom-right corner. Word XP and later offer an Open And Repair option, which you can use to force Word to attempt a recovery. Note that this method is limited to documents in the Word 97-2003 format (not docx or dotx files). You will lose Word formatting and nontext items such as graphics, but you should at least be able to extract most of the text information from the file. This filter will import straight ASCII text from any file. ![]() Then, choose Recover Text From Any File from the Files Of Type drop-down list and open the document. When the Open dialog box appears, select the troublesome Word document. You may also be able to recover the text portion of a document using Word’s text converter. Alternatively, you can use a file editor (a hex editor) to manually extract anything salvageable from the file. One approach is to use another word processor that supports Word documents. ![]() 6: Extract the raw textĪlthough saving the Word document as an RTF or TXT file usually works pretty well, that technique does you no good if Word won’t open the file. Sometimes, using a format such as RTF or TXT will cause some of Word’s codes to be stripped from the document, which often fixes the problem. If you can open the file in Word, try saving the file in an alternate format. If nothing else, running CHKDSK allows you to test the integrity of the file system, which allows you to determine whether the problem exists at the file system level or within the document itself. Your problem could be caused by corruption at the file system level, and CHKDSK may be able to fix the problem. Try running CHKDSK against the volume containing the corrupted file. Try selecting the Sent Items folder and using Outlook’s Search feature to look for the document’s name. If so, a copy of the document may still be in Outlook’s Sent Items folder, attached to the email message you sent. In many cases, you may have emailed a copy of the document to someone at some point. Making a backup copy of the file now may save you even more heartache later on. There’s a chance that the corruption within the file could become worse over time or that you could destroy the file while trying to recover it. If you don’t have a backup copy of the corrupt document readily available, make a copy of the corrupted file right away and store it on removable media. This isn’t really a recovery trick, but it is a crucial preliminary step. Try opening your “corrupt” document from another computer. Before you start trying to restore a backup or repair a file, try opening other documents. It could be Word or even Windows that is having the problem. LEARN MORE: Office 365 Consumer pricing and features 1: Don’t assume that the document is corruptĪutomatically assuming that a document is corrupt can be a big mistake. Note: This article is also available as a PDF download. Here are some things you can try when you’re dealing with a corrupted Word document. But hang on: You may not have to accept data loss. You’ve lost critical information and/or countless hours of work – or so it appears. If you’ve ever had an important document get corrupted, you know the despair that sets in. TIOBE Index: Top 10 Most Popular Programming Languages Top Tech Conferences & Events to Add to Your Calendar IBM, ISC2 Offer Cybersecurity Certificate But before you resign yourself to losing the document contents, check out these techniques for salvaging your text. ![]() Few computer experiences are worse than having a Word document blow up on you. ![]() ![]() ![]() The above mentioned are two of the most common Garageband for PC alternatives. The data is stored in the cloud so that you can access your personal music data anywhere across any devices that you own. Audiotool: Yet another popular Garageband for PC alternative, Audiotool is an online tool that helps you create awesome music.This software is available as a free trial and also as $90 premium software. There is an official version of Mixcraft available for all the latest Windows OS versions and also features all the awesome features of Garageband. Mixcraft: Mixcraft is a really popular Garageband for PC alternative.There are many Garageband for PC alternatives out there. So devices like the Apple iPhone, iPad and Mac powered machines can run the Garageband software easily without any hassles. As the Garageband software is developed by Apple Inc., it is exclusive to devices powered by the MacOS and iOS. Is there an Official Version of Garageband for PC/Windows 10, 7, 8?ĭespite being one of the best and most popular Digital Audio Workstation software out there, sadly there is no official version of Garageband for PC/Windows 10, 7 or 8. The main attraction with the Garageband software was its simplicity, like pretty much every other software products developed by Apple. Just after the launch of Garageband, the software became a huge success and people who owned a Mc and later an iOS device started using Garageband to create podcasts or music tracks etc. ![]() and was initially available for their Mac operating system and was later ported to the iOS operating system once iOS became popular. The software is developed and released by Apple Inc. The Garageband Digital Audio Workstation software is one of the best such tool available out there. Despite lacking some of the cutting edge features of its competitors, Garageband is still considered one of the best Digital Audio Workstation software out there and today we are here with a detailed tutorial on how to download Garageband for PC (Windows 10, 7, 8) featuring the latest update for the software. However, not any single Digital Audio Workstation software is capable of impressing everyone who works with such software, with the exception of “Garageband”. ![]() Many among them are free whereas others are paid ones. There is a lot of Digital Audio Workstation software available out there. ![]() ![]() If you liked the article please share it. Note: Your phone should be connected to network if you want to use the app on Windows 7. ➟ Check this article to install WhatsApp, WhatsApp Business on Windows 10. To logout from the app just click the three dots on the top left corner next to a plus sign and click ' Log Out'.The WhatsApp desktop application will be activated and you can chat, share files from the computer's hard disk.From your smartphone scan the QR code that is appearing on your computer's screen.Now on your smartphone open WhatsApp and go to " Menu>WhatsApp Web".Make sure that your desktop and smartphone should be connected to internet.After installation a windows will open automatically showing the random QR codes.Download the application for Windows 8 64-bit or 32-bit according to your desktop computer's OS architecture.How To Install WhatsApp On Windows 7 64-bit And 32-bit PC To make it work you need to scan the QR code from your WhatsApp on your smartphone. The standalone application is an implementation of the whatsapp web app in which you no longer need to use the browser. How Does The WhatsApp Windows 7 App Works All you have to do is to download and install it on your PC or laptop or notebook. Send any file type in WhatsApp īut there's a good news as the standalone application for Windows 8 works on Windows 7 64-bit too. ![]() The developers have not developed any standalone application for the Windows 7 ultimate 64-bit and 32-bit PC. It is also available for MAC OSX Mavericks v10.9 or higher. This can be installed on Windows 10 also. Generally, the process takes 3-4 minutes. Click Install to initiate the download and install process. ![]() In the search bar present at the top of the window, type WhatsApp and select the appropriate result. Press the Windows key, type microsoft store and click Open. ![]() If you use this app mostly on your phone then you may not be aware of that recently WhatsApp standalone desktop application was released for the Windows 8 64-bit and 32-bit PC/Computer. Use Microsoft Store to download & install. 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The images are either royalty free or distributed widely on the Internet, and they are of unknown origin for the most part. Home printer Free Airplane Coloring Pages These airplane coloring sheets are a great way to enhance fine motor skills. Each page is an invitation to unlock your imagination and infuse it. As you color in the pages, you’ll have the chance to recreate the enchanting skyline, add your flair to Central Park’s serene beauty, or even bring the dazzling lights of Times Square to life. Robert Pondiscio, a teaching expert and senior fellow at the American Enterprise Institute, weighed in on the backlash, saying he wasn’t convinced the book was an attempt to indoctrinate kids. New York Buffalo Note: All coloring pages are offered free of charge and for personal use only. Our New York coloring pages let you immerse yourself in this vibrant tapestry. 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That’s the format most resumes you’ve seen follow - its main focus is your work experience, written down in reverse-chronological order. In 99% of the cases, you’ll want to go with the Reverse Chronological resume format. If you want to personalize your resume a bit more, you can also include the following sections:įor more information on how to place these sections on your resume, check out our article on what to put on a resume. Instead, you should focus on what you DO have: education, projects, volunteering experience, hobbies & interests.įor a complete guide on how to make a resume with no work experience stand out, check out our article. If you’re applying for an entry-level job or an internship, no one expects you to have ANY work experience. So, what the heck can you include in your resume if you have none? After all, most resume examples you see on the web are 80% about work experience. The process of writing a resume might seem super scary to you. ![]() How to Write a Resume for Your First Job? Want to learn how? Check out our complete guide on how to make a resume. If you manage to create a “good” resume, you’re going to 2x your chances of getting hired. When applying for a job, you’re (in most cases) going to be asked for a resume accompanied by a cover letter. Its main purpose is to show off your best self to potential employers. A resume is a brief summary of personal and professional experiences, skills, and education history. ![]() ![]() ![]() All you have to do is open the spreadsheet as soon as you enter the gym, and you will know exactly what to do. This eliminates the need to remember what muscle group you have to work on that day. You can also create columns targeting specific muscle groups per day. The data will be presented in columns, and you can sort all the data by date so you know exactly what you did on a specific date. 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